Winemaking generates different biomolecule-rich byproducts, including pomaces (skin and seeds), lees, as well as other solid wastes like grape canes. Among them, grape wood biomass, which are discarded after winter pruning, represent great potential for the development of new natural cosmetic ingredients due to a large abundance and the presence of polyphenols including stilbenoids. It is assumed that the polyphenol content in grapevines might change according to genotypes and environmental parameters. Considerable advances have been made in the chemical characterization of wines, particularly volatiles and polyphenolic compounds using advanced metabolomics tools. However, the metabotyping of grapevine organs, i.e. the characterization of metabolic phenotypes, is very limited. Analytical methods using Ultra High Performance Liquid Chromatography (UPLC) in tandem with Diode Array Detection (DAD) and Mass Spectrometry (MS) in combination with chemometric analyses have been developed offering rapid and comprehensive metabolomic analyses of grape chemical composition. We explored the metabolic variations of polyphenol metabolism depending on varietal diversity and how these variations influence the cosmetic potential of the resulting natural ingredients.