Atomic force microscopy (AFM) is a tool for nanoscale analysis-based approach allowing to obtain mechanical information about structures. Although it is basically used for the characterization of stiff materials, AFM can be used to extract mechanical and structural properties of biological samples. Indeed, in addition to biologically answer to a stimulation, biological samples also respond to a biomechanically manner as for example in the case of the hydration process. At BioMeca ®, we used this approach to characterize among other things, the effects of active ingredients on biological samples such as skin, to link and highlight biological and mechanical responses after application. Coupled to fluorescence approaches, AFM is a powerful tool to characterize products in cosmetic and pharmacological field.